Dorsey, John G. Department of Chemistry, University of Cincinnati, Cincinnati, Ohio.
Last reviewed:June 2018
Show previous versions
- Separation modes
- Ion exchange
- Size exclusion
- Bonded phases
- Normal and reversed phase
- Hydrophobic interaction
- Affinity chromatography
- Links to Primary Literature
- Additional Readings
A method of chemical separation that involves passage of a liquid phase through a solid phase and relies on subtle chemical interactions to resolve complex mixtures into pure compounds. A small amount of the sample to be separated is injected onto the top of a column that is densely packed with spherical particles of small diameter; that is, the stationary phase. A liquid solvent, the mobile phase, flows through the column continuously to carry the sample from the top to the bottom of the column. During passage through the column, the components of the sample are transferred back and forth continuously between the two phases, and small thermodynamic differences in the chemical interactions of the various sample components with the mobile and stationary phases slow the passage of some solutes more than others and lead to their separation. The technique can be performed on very small scales for chemical analysis, dealing with micrograms or even nanograms of sample, or it can be performed on an industrial scale for purification of commercial products. The technique has great resolving power (see illustration). See also: Chromatography
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Contributors include more than 9000 highly qualified scientists and 43 Nobel Prize winners.
MORE THAN 8700 articles covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 19,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information