Fluorescence microscopy: high-resolution methods
Nägerl, Urs Valentin Bordeaux Neurosciences Institute, Université Victor Segalen Bordeaux 2, Bordeaux, France.
- STED microscopy
- PALM and STORM
- Links to Primary Literature
- Additional Readings
Fluorescence microscopy is one of the most powerful and widely used imaging techniques in biological research because it can visualize dynamic processes inside living cells with exquisite sensitivity and specificity. Major breakthroughs have included the development of confocal microscopy in the 1980s and two-photon microscopy in the 1990s. These advances have been accompanied by groundbreaking improvements in the ability to visualize specific proteins and organelles inside living cells using fluorescent proteins [for example, green fluorescent protein (GFP)] and transgenic technology. In addition, tremendous increases in computing power have enabled the acquisition and analysis of large imaging data sets and thus have further facilitated the ascendancy of fluorescence microscopy.
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Contributors include more than 9000 highly qualified scientists and 43 Nobel Prize winners.
MORE THAN 8700 articles covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 19,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information