Ploem, Johan S. Laboratorium voor Cytochemie en Cytometrie, Rijksuniversiteit Leiden, Wassenaarseweg, The Netherlands.
- Microscope components
- Confocal laser-scanning fluorescence microscopy
- Links to Primary Literature
- Additional Readings
An instrument for the observation and study of microscopic specimens that absorb light and emit fluorescence. The ability of substances to emit light when excited by a strong light source is called luminescence, which encompasses the phenomena of phosphorescence and fluorescence. If the secondary emission continues for some time (milliseconds) after the light source has been turned off, it is referred to as phosphorescence. The ability to emit light only very briefly (nanoseconds) after the absorption of light is termed fluorescence. Many specimens obtained from plants, animals, and minerals have the ability to emit a weak to strong fluorescence when excited with appropriate light and without being specifically treated or stained. That phenomenon is called autofluorescence. In most cases, in order to obtain specific and meaningful fluorescence, staining with fluorescing dyes called fluorophores or fluorochromes is necessary. Fluorescence microscopy is a highly sensitive method, since often minute quantities of a fluorophore can be visualized with good microscopic contrast. In appropriate applications, brightly fluorescing images can be observed against a dark background. Individual fluorophores have different absorption and emission spectra and a different quantum efficiency (the ratio between the energy absorbed and the energy emitted), factors that must be considered for optimum fluorescence. See also: Bioluminescence; Fluorescence; Luminescence; Phosphorescence
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Contributors include more than 9000 highly qualified scientists and 43 Nobel Prize winners.
MORE THAN 8700 articles covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 19,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information