Somlyo, Andrew Paul Department of Physiology, School of Medicine, University of Virginia, Charlottesville, Virginia.
- X-ray detection and data processing
- Quantitative analysis
- X-ray mapping
- Specimen preparation by rapid freezing
- Biological applications
- Related Primary Literature
- Additional Reading
A method used for determining the elemental composition of materials, based on the x-rays emitted by different elements when bombarded with high-energy electrons. It is a micro method that can detect x-ray photons emitted by the atoms within a small volume excited by an electron beam focused to 10 nanometers diameter or less. In biology, electron-probe microanalysis can be used to determine the composition of cell organelles without isolating them and therefore altering the distribution of diffusible elements. Studies with electron-probe analysis have thrown light on the major problems of excitation-contraction coupling in muscle, visual transduction in retinal rods, and the functions of the endoplasmic reticulum and mitochondria in the regulation of cytoplasmic calcium.
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