Novikoff, Phyllis M. Department of Pathology, Albert Einstein College of Medicine, Bronx, New York.
Last reviewed:December 2019
- Enzyme cytochemistry
- In situ hybridization
- Lectin cytochemistry
- Fluorescence analog
- Measuring pH
- Marker enzymes
- Epitope-tagged proteins
- Histologic dyes
- Laser scanning confocal microscopy
- Specimen preparation
- Related Primary Literature
- Additional Reading
The science concerned with the chemistry of cells and cell components, with particular emphasis on the location of chemical constituents and enzymes. Specifically, for the purposes of cytochemistry, the macromolecules of subcellular structures can be treated chemically to form microscopically visible end products. For example, proteins, enzymes, lipids, carbohydrates and nucleic acids can be directly visualized in cell nuclei, membranes and organelles by cytochemical methods that generate images that can be viewed with bright-field, light, confocal, electron, or fluorescence microscopes (see illustration). See also: Cell (biology); Cell, spectral analysis of; Confocal microscopy; Electron microscope; Fluorescence microscope; Interference microscope; Phase-contrast microscope; Polarized light microscope; Scanning electron microscope
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