CRISPR genome-editing methods against superbugs
Waikel, Rebekah L. Department of Biological Sciences, Eastern Kentucky University, Richmond, Kentucky.
- Adaptive immune system in humans and microbes
- CRISPR-Cas adaptive immune system
- Antimicrobial CRISPR-Cas system
- Links to Primary Literature
- Additional Readings
Superbugs—bacterial strains that are resistant to several types of antibiotics—have become an increasingly serious health concern. The Centers for Disease Control and Prevention (CDC) in the United States reports that at least 2 million Americans become ill with antibiotic-resistant infections annually, with more than 23,000 of these cases resulting in death. Resistance results from the overuse and misuse of antibiotics and from the ability of the infecting bacteria to acquire resistance genes, typically through horizontal transfer (the passing of genetic material from one organism to another organism, either related or unrelated, through nonreproductive mechanisms). One of the most well known and most common superbugs is methicillin-resistant Staphylococcus aureus (MRSA). The CDC estimates an incidence of 80,000 aggressive cases of MRSA per year, with 11,000 of those resulting in death. The emergence of bacterial resistance to methicillin and other beta-lactam antibiotics has prompted the use of carbapenems, which are typically used only as a last-resort drug. It is not surprising that the bacterial family of carbapenem-resistant Enterobacteriaceae (CRE) is now one of the greatest threats to human health, with mortality rates approaching 50% according to the CDC. The increases in cases of MRSA and CRE have prompted the need to find alternative antimicrobial approaches. One promising antimicrobial tool is the use of CRISPR-Cas (clustered regularly interspaced short palindromic repeats and associated proteins) genome editing to either kill resistant bacteria or eradicate antibiotic resistance and virulence genes.
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Contributors include more than 9000 highly qualified scientists and 43 Nobel Prize winners.
MORE THAN 8700 articles covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 19,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information