Joenje, H. Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands.
Levitus, Marieke Department of Clinical Genetics, VU University Medical Center, Amsterdam, The Netherlands.
- Complementation analysis
- Complementation groups
- Complementation cloning
- Intragenic complementation
- Links to Primary Literature
- Additional Readings
The correction of a genetic defect through the supply of a missing component. Complementation occurs when a normal copy of a gene is introduced into a cell that harbors a defective copy of that gene. If several genes collaborate to support a certain function (for example, to form a structure), cell crossings (somatic cell hybridization) may be used to determine the number of genes involved. Two cells, upon crossing, typically complement each other's defect if their mutations are in different genes, whereas the defect persists if the mutations are in the same gene. Special mouse cell lines have been developed that harbor single human chromosomes, which can be introduced into a human cell line by microcell-mediated chromosome transfer. This technique allows for the identification of the chromosome that harbors a complementing gene. See also: Chromosome; Gene; Genetics; Mutation
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Contributors include more than 9000 highly qualified scientists and 43 Nobel Prize winners.
MORE THAN 8700 articles covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 19,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information