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Search Results: Yeast functional analysis

Analysis of the genome of Saccharomyces cerevisiae (brewer's and baker's yeast) has provided an opportunity for developing new tools for the...



= Encyclopedia Article; = Research Update
Figure 1.Two analytic techniques. (a) Short flanking homology–PCR technique. The disruption cassette generated consists of a marker DNA flanked by small homology regions to the target gene. (b) Long flanking homology–PCR technique. The disruption cassette generated consists of a marker DNA flanked by long homology regions to the target gene. (After A. Wach et al., Guidelines for EUROFAN B0 Program)
Figure 2.Schematic view of the construction of the cognate gene clone by gap repair in yeast. (After A. Wach et al., Guidelines for EUROFAN B0 Program)
Figure 3.Transposon mutagenesis. (a) Structure of mTns transposons, and excision after Cre expression. (b) Construction of the library insertion mutant.