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Search Results: Capillary electrophoresis-SELEX

Every chemical reaction or biochemical pathway is influenced by how molecules interact with each other. Considering this, it is not surprising that...


= Encyclopedia Article; = Research Update
Figure 1.CE-SELEX process used to isolate the ssDNA sequences with high affinity for a target molecule. A random-sequence ssDNA library is incubated with the target molecule. The incubation mixture is separated using CE where ssDNA that bind the target migrate through the capacity at a different velocity than nonbinding sequences, allowing them to be isolated. Binding sequences are PCR-amplified, purified, and made single-stranded, generating a new ssDNA pool ready for further rounds of enrichment. High-affinity aptamers are typically obtained after two to four rounds of CE-SELEX selection.
Figure 2.Secondary structure predicted by mFOLD of a ssDNA aptamer selected using CE-SELEX with affinity for neuropeptide Y. mFOLD is a readily available algorithm for predicting nucleic acid secondary structure.
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