Embryonated egg culture
Melnick, Joseph L. Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas.
- Additional Readings
The process of cultivating embryonated hens' eggs inoculated with animal viruses for scientific purposes, including the production of viral vaccines. Embryonated eggs are among the most useful and available forms of living animal tissue for the isolation and identification of animal viruses, for titrating viruses, and for quantity cultivation in the production of viral vaccines. The embryo proper, chorioallantoic membrane, yolk sac, allantoic sac, or amniotic sac may be inoculated in hens' eggs of various ages, so a wide choice of types of tissue is available to fit the characteristics of the virus under study or for special investigations. The chorioallantoic membrane is frequently used; in some infections, such as smallpox, vaccinia, and herpes simplex, characteristic lesions are produced, which in some cases may resemble those in the natural host. For example, smallpox virus when cultured on the chorioallantoic membrane produces pocks and typical inclusions within the infected cells. When the embryo is inoculated, characteristic skin eruptions appear. Influenza virus, however, when inoculated into the amniotic cavity, does not give rise to pathology like that of the natural infection. See also: Herpes; Influenza; Smallpox
The content above is only an excerpt.
for your institution. Subscribe
To learn more about subscribing to AccessScience, or to request a no-risk trial of this award-winning scientific reference for your institution, fill in your information and a member of our Sales Team will contact you as soon as possible.
to your librarian. Recommend
Let your librarian know about the award-winning gateway to the most trustworthy and accurate scientific information.
AccessScience provides the most accurate and trustworthy scientific information available.
Recognized as an award-winning gateway to scientific knowledge, AccessScience is an amazing online resource that contains high-quality reference material written specifically for students. Its dedicated editorial team is led by Sagan Award winner John Rennie. Contributors include more than 9000 highly qualified scientists and 42 Nobel Prize winners.
MORE THAN 8500 articles and Research Reviews covering all major scientific disciplines and encompassing the McGraw-Hill Encyclopedia of Science & Technology and McGraw-Hill Yearbook of Science & Technology
115,000-PLUS definitions from the McGraw-Hill Dictionary of Scientific and Technical Terms
3000 biographies of notable scientific figures
MORE THAN 17,000 downloadable images and animations illustrating key topics
ENGAGING VIDEOS highlighting the life and work of award-winning scientists
SUGGESTIONS FOR FURTHER STUDY and additional readings to guide students to deeper understanding and research
LINKS TO CITABLE LITERATURE help students expand their knowledge using primary sources of information